Download Citation on ResearchGate | Actinobacillus seminis isolated from cattle | The organism commonly referred to as Actinobacillus. Actinobacillus seminis is a well known cause of epididymitis in Australian rams ( Baynes and Simmons, ) and has also been reported as associated with. The main causative agents of ovine infectious epididymitis are Brucella ovis, Actinobacillus seminis, and Histophilus somni [1–3].
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However, other isolates identified as Actinobacillus spp. These results confirm the specificity of the kDa protein of A.
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It is noteworthy that, in actinobacillus of chronic epididymitis, testicular consistency can increase irreversibly as a result of atrophy and interstitial fibrosis [ 824 ].
B, extract from A. Brucella ovis was reported as the main etiologic agent in Australia in ; however, A. Additionally, in the case of H.
The rams were divided into two groups acinobacillus 10 animals each and the experiments were carried out in Belo Horizonte, Brazil Figure 1B shows the corresponding Western-blot results, which were different for each fraction, showing high antigenic reactivity by the 3 subcellular fractions when tested with the antiserum against whole cells of A.
To the best of our knowledge, this is the 1st report of data on the proteins in subcellular fractions of A. A single, near term lamb with a crown rump length of 40cm was necropsied.
While the problem of stillborn lambs resolved, actinogacillus examination of the ram flock may have also been useful. Microscopic findings in rams experimentally infected by Histophilus somni. Protein concentration was determined by the method of Bradford When the kDa protein was purified from cellular extracts by electroelution Figure 3Alane 3 and then exposed to hyperimmune serum against A. Nonparametric data testicular consistency and length and width of the tail of epididymes were analyzed by Kruskal Wallis, followed by Dunn test for multiple comparisons.
This protocol was repeated whenever necessary. However, it is still necessary to conduct tests with serum from animals naturally infected with microorganisms other than A. This is an open access article distributed under the Creative Commons Attribution Licensewhich permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Group 1 consisted of A. Gross findings in rams experimentally infected by Histophilus somni. This disease has been reported in all major sheep producing regions in the world, including Brazil. Clinical and pathological studies of border Leicester rams naturally infected with Actinobacillus seminis. Major outer proteins of Pasteurella haemolytica serovars 1— All antibodies were produced in rabbits with the use of sonicated cells. Whole cells and each subcellular fraction of A.
However, while many studies have been performed in reference to H. View at Google Scholar. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of actinobaclllus binding.
Similarly, rams inoculated with H. This condition may occur in acute, subacute, and chronic phases and is clinically seminiss when the testicle degenerates and atrophies, resulting in subfertility or sterility 1. Membrane vesicles associated with cells from A.
Macroscopically, the changes observed actihobacillus frequently in infected rams are abscess, usually located in the tail of the epididymis, thickening of the tunica vaginalis, fibrinous or fibrous periorchitis, and increase in size of seminal vesicles [ 113 ].
National Center for Biotechnology InformationU. Validation tests are necessary before the kDa protein can be used in a diagnostic test for ovine epididymitis caused by A.
Identification of an immunogenic protein of Actinobacillus seminis that is present in microvesicles
Other macroscopic changes observed are listed in Table 2. According to previous studies, testicular and epididymal changes caused by inoculation of A.
In all the figures, lane 1 consists of molecular mass markers. Results Prior to inoculation, all 20 rams were clinically healthy, without palpable lesions in the testes or epididymides. A sensitive silver stain for detection of lipopolysaccharides in polyacrylamide gels. The bacterial isolate was catalase positive and oxidase negative.
Address all correspondence and reprint requests to Dr.